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Ten polymorphic microsatellite loci for the Atlantic halibut (Hippoglossus hippoglossus) and cross-species application in related species |
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| Authors: | Hao Ding Changwei Shao Xiaolin Liao Genbo Xu Xiangshan Ji Songlin Chen |
| Institution: | (1) Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, 266071 Qingdao, China;(2) College of Aqua-Life Science and Technology, Shanghai Fisheries University, Shanghai, 266003, China |
| Abstract: | In the present study, 10 polymorphic microsatellite DNA loci from Atlantic halibut (Hippoglossus hippoglossus) were isolated and characterized. The number of alleles for these loci ranged from 2 to 4 in tested 24 individuals. Observed and expected heterozygosities per locus varied from 0.21 to 0.70 and from 0.31 to 0.65, respectively. Most of these 10 microsatellite loci were successfully amplified and showed polymorphic in five related species. These loci will be useful for the assessment of genetic diversity and population structure of Atlantic halibut. H. Ding and C. Shao contributed equally to this work. |
| Keywords: | Atlantic halibut Hippoglossus hippoglossus Microsatellite markers Polymorphism |
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