Site-specific recombination in Arabidopsis plants promoted by the Integrase protein of coliphage HK022 |
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| Authors: | Pnina?Gottfried,Ofra?Lotan,Mikhail?Kolot,Ludmila?Maslenin,Rosa?Bendov,Rena?Gorovits,Vered?Yesodi,Ezra?Yagil mailto:yagill@post.tau.ac.il" title=" yagill@post.tau.ac.il" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author,Arie?Rosner |
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| Affiliation: | (1) Department of Biochemistry, Tel-Aviv University, Tel-Aviv, 69978, Israel;(2) FertiSeeds Ltd., 13A Building, K. Weizmann Science Park, Ness Ziona, 70400, Israel;(3) Department of Virology, Agricultural Research Organization, The Volcani Center, Beit Dagan, 50250, Israel;(4) Department of Plant Pathology and Microbiology, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, 76100, Israel |
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| Abstract: | ![]()
The gene encoding the wild type Integrase protein of coliphage HK022 was integrated chromosomally and expressed in Arabidopsis thaliana plants. Double-transgenic plants cloned with the int gene as well as with a T-DNA fragment carrying the proper att sites in a tandem orientation showed that Int catalyzed a site-specific integration reaction (attP × attB) as well as a site-specific excision reaction (attL × attR). The reactions took place without the need to provide any of the accessory proteins that are required by Int in the bacterial host. When expressed in tobacco plants a GFP-Int fusion exhibits a predominant nuclear localization. These authors contributed equally to this work |
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| Keywords: | Arabidopsis Integrase nuclear localization phage HK022 site-specific recombination |
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