Restriction fragment alleles of the rabbit IGHG genes with reference to the rabbit IGHGCH2 or e locus polymorphism |
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Authors: | Esteves P J Alves P C Ferrand N van der Loo W |
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Affiliation: | Centro de Estudos de Ciência Animal, ICETA-UP, Campus Agrário de Vair?o, Portugal. |
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Abstract: | ![]() Among domesticated mammals, rabbit (Oryctolagus cuniculus) is the only species possessing not more than one subclass of immunoglobulin (IgG) antibodies. The rabbit IGHGCH2 or e locus presents two serologically defined alleles, the e14 and e15 allotypes, which are correlated with amino acid variation at the IgG CH2-CH3 interface. Genetic studies, while revealing the adaptive value of this polymorphism, have relied so far entirely upon allo-antisera. Here we show how these alleles can be distinguished by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods. The proposed PCR-RFLP approach allows the monitoring of IGHG locus diversity in rabbit. |
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Keywords: | allotypes antibody e locus IGHGCH2 locus PCR–RFLP polymorphism rabbit |
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