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Restriction fragment alleles of the rabbit IGHG genes with reference to the rabbit IGHGCH2 or e locus polymorphism
Authors:Esteves P J  Alves P C  Ferrand N  van der Loo W
Affiliation:Centro de Estudos de Ciência Animal, ICETA-UP, Campus Agrário de Vair?o, Portugal.
Abstract:
Among domesticated mammals, rabbit (Oryctolagus cuniculus) is the only species possessing not more than one subclass of immunoglobulin (IgG) antibodies. The rabbit IGHGCH2 or e locus presents two serologically defined alleles, the e14 and e15 allotypes, which are correlated with amino acid variation at the IgG CH2-CH3 interface. Genetic studies, while revealing the adaptive value of this polymorphism, have relied so far entirely upon allo-antisera. Here we show how these alleles can be distinguished by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods. The proposed PCR-RFLP approach allows the monitoring of IGHG locus diversity in rabbit.
Keywords:allotypes    antibody    e locus    IGHGCH2 locus    PCR–RFLP    polymorphism    rabbit
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