A monoclonal antibody against T-cell receptor αβ induces endogenous cytokines and prevents mice from a lethal infection with Listeria monocytogenes |
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Authors: | Akio Nakane Shinsuke Nishikawa Sanae Sasaki Tomisato Miura Misako Asano Masashi Kohanawa Tomonori Minagawa |
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Institution: | Department of Bacteriology, Hirosaki University School of Medicine, Zaifu-cho 5, Hirosaki 036, Japan;School of Allied Medical Sciences, Hirosaki University, Hon-cho 66-1, Hirosaki 036, Japan;Department of Microbiology, Hokkaido University School of Medicine, Kita 15 Nishi 7, Kita-ku Sapporo 060, Japan |
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Abstract: | Abstract In vivo induction of cytokines by a monoclonal antibody (mAb) against T-cell receptor (TCR) αβ and the protective effect induced by the mAb on a lethal infection with Listeria monocytogenes were studied. Injection of anti-TCR αβ mAb induced rapid production of endogenous tumour necrosis factor in the spleens, and gamma interferon and interleukin-6 in the bloodstreams and spleens of mice. Administration of anti-CD4 mAb, anti-CD8 mAb, or anti-Thy1.2 mAb resulted in suppression of anti-TCR αβ mAb-induced endogenous cytokine production. Mice were protected against lethal L. monocytogenes infection when treated with anti-TCR αβ mAb. The protective effect was not demonstrated in CD4 + cell- or CD8 + cell-depleted mice. These results suggest that anti-TCR αβ mAb shows a protective effect on a lethal infection with L. monocytogenes in mice and that the mAb-induced endogenous cytokines might be involved in the effect of anti-TCR αβ mAb. |
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Keywords: | Listeria monocytogenes T-cell receptor αβ Gamma interferon Tumour necrosis factor Interleukin-6 |
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