Transformation of plant mitochondria with mitochondrial DNA plasmids via protoplast fusion |
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Authors: | Roger J Kemble Tina L Barsby and Stephen A Yarrow |
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Institution: | (1) Allelix Inc., 6850 Goreway Drive, L4V 1P1 Mississauga, Ontario, Canada;(2) Present address: Nickerson Seed Co. Ltd., Cambridge Science Park, Milton Road, CB4 4WE Cambridge, UK |
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Abstract: | Summary
Brassica napus cybrid plants which contain novel nucleus-mitochondria-chloroplast combinations have been constructed, via protoplast fusion. Such fusions resulted in mitochondrial DNA plasmids being lost (at a frequency of 12.5%) or, more surprisingly, being transferred from mitochondria of one protoplast population to mitochondria of the other population (at a frequency of 6.1%). Mitochondria containing their new DNA complement became the dominant organelle population in regenerated plants and were faithfully maternally inherited through successive sexnal generations. No concomitant alterations in mitochondrial chromosome organization or nuclear chromosome number occurred. Protoplast fusion can, therefore, cure plant mitochondria of extrachromosomal DNA and, more importantly, be used to transform plant mitochondria with naturally occurring mitochondrial plasmids. The potential for mitochondrial transformation with recombinant vectors is discussed. |
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Keywords: | Brassica Protoplast fusion Mitochondrial DNA Plasmids Transformation |
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