| 红色毛癣菌菌株的特异性PCR鉴定方法研究 |
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| 引用本文： | 费实,高爱莉,黎小东,李振洁,肖常青,朱慧兰.红色毛癣菌菌株的特异性PCR鉴定方法研究[J].中国真菌学杂志,2010,5(5):282-285. |
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| 作者姓名： | 费实 高爱莉 黎小东 李振洁 肖常青 朱慧兰 |
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| 作者单位： | 广州市皮肤病防治所,广州,510095 |
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| 摘 要： | 目的建立一种快速的红色毛癣菌分子生物学鉴定方法。方法根据红色毛癣菌保守区域-真菌核糖体DNA（rDNA）的转录间隔区（ITS）设计特异性引物,采用上游：ITS19865＇GAC ACC AAG AAA AAA TTC TCT GAA GA3＇,下游：ITS24415＇GTC CTG AGG GCG CTG AA3＇为引物对45株红色毛癣菌、5株须癣毛癣菌和1株紫色毛癣菌菌株的DNA进行PCR扩增,观察产物电泳带型的差异。结果 45株红色毛癣菌均能扩增出目的片段,5株须癣毛癣菌和1株紫色毛癣菌均无目的片段扩增出。结论红色毛癣菌可用特异引物PCR方法快速鉴定。
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| 关 键 词： | 红色毛癣菌 PCR 须癣毛癣菌 皮肤癣菌 |
PCR identification of Trichophyton rubrum |
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| FEI Shi,GAO Ai-li,LI Xiao-dong,LI Zhen-jie,XIAO Chang-qing,ZHU Hui-lan.PCR identification of Trichophyton rubrum[J].Chinese JOurnal of Mycology,2010,5(5):282-285. |
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| Authors: | FEI Shi GAO Ai-li LI Xiao-dong LI Zhen-jie XIAO Chang-qing ZHU Hui-lan |
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| Institution: | (Guang zhou Institute of Dermatology and Venerology,Guang zhou 510095,China) |
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| Abstract: | Obiective To develop a rapid method to for identification of Trichophyton rubrum.Methods Primed ITS1986 5＇GAC ACC AAG AAA AAA TTC TCT GAA GA3＇,ITS2441 5＇GTC CTG AGG GCG CTG AA3＇ were chosen to amplify 45 strains of Trichophyton rubrum,5 strains of Trichophyton mentagrophytes and 1 strain of Trichophyton violaceum.Results Characteristic bands were amplified in Trichophyton rubrum.But there was no characteristic band found in Trichophyton mentagrophytes and Trichophyton violaceum.Conclusions Trichophyton rubrum could be identificated by PCR rapidly. |
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| Keywords: | PCR |
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