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Rice bran extract affects differentiation of mesenchymal stem cells potency into osteogenic cells
Authors:Ken Fukumoto  Takuo Tsuno  Masayuki Taniguchi  Satoshi Terada
Affiliation:1. Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, 3-9-1, Bunkyo, Fukui, 910-8507, Japan
2. Tsuno Food Industrial Co., Ltd, 94 Shinden, Katsuragi-Cho, Ito-Gun, Wakayama, 649-7194, Japan
3. Department of Materials Science and Technology, Graduate School of Science and Technology, Niigata University, 2-8050 Ikarashi, Nishi-ku, Niigata, 950-2181, Japan
Abstract:As rice bran contains various nutrients and other proteins of which a part has biological effects on animal cells, we tested the effect of rice bran extract on rat mesenchymal stem cells (rMSCs) obtained from bone marrow. These rMSCs are pluripotent and can be readily induced to differentiate into a number of cell types, including bone and cartilage. rMSC was aggregated by culturing in serum-free condition with rice bran extract, but was not aggregated by culturing in serum-free condition or in serum-containing medium. Moreover, the longer aggregates of rMSCs were cultured in serum-free condition with rice bran extract, the more the aggregates grew. After two passages in serum-free conditions, rMSCs lost their potency for differentiation into osteogenic cells; however, the addition of rice bran extract to serum-free medium successfully prevented the loss of this ability for differentiation. In addition, MSC makers CD105 and CD166 gene expression in serum-free condition with rice barn extract corresponded to these expressions in serum-containing medium. This result suggests that certain factors in rice bran could be bioactive and contribute toward retaining the ability of MSCs to differentiate into osteogenic cells after passaging.
Keywords:Mesenchymal stem cell (MSC)   Rice bran extract   Serum-free medium   Osteogenic differentiation
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