|
|||||
|
|
Emergence and dissemination of Enterobacteriaceae with plasmid-mediated CMY-6 and CTX-M-15 beta-lactamases in community in North-India |
|
| Authors: | M Shahid V M Ensor P M Hawkey |
| Institution: | 1. Section of Antimicrobial Resistance Researches and Molecular Biology, Department of Microbiology, Jawaharlal Nehru Medical College & Hospital, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, India 2. Antimicrobial Agents Research Group, Institute of Biomedical Research, University of Birmingham, Vincent Drive, Edgbaston, Birmingham, B15 2TT, UK 3. West Midlands Health Protection Agency, Heart of England NHS Foundation Trust, Bordesley Green East, Birmingham, B9 5SS, UK |
| Abstract: | A collection of 88 Escherichia coli and 22 Klebsiella pneumoniae strains previously studied for the carriage of bla CTX-M-15 were screened for the presence of bla ampC using multiplex PCR, and analysed for antibiotic resistance and co-carriage of bla ampC and bla CTX-M-15. Cefoxitin resistance was noticed in total of 41.8% isolates. Concomitant resistance to third and fourth generation cephalosporins, aztreonam, gentamicin, trimethoprim and ciprofloxacin was noted. A total of 20% (22/110) isolates (14.5% E. coli and 5.5% K. pneumoniae) carried plasmidic bla ampC of the CIT-family. Sequencing of the representative isolates revealed CMY-6 AmpC-beta-lactamase. Simultaneous occurrence of bla ampC and bla CTX-M was noticed in 77.3% (17/22) isolates. Plasmid analysis of the selected isolates showed the presence of a single plasmid of ca. ~23 kb. Curing and transconjugation experiments provided evidence of carriage of bla CMY and bla CTX-M on same plasmid. RAPD typing revealed the presence of bla CMY and bla CTX-M in a wider range of strains of E. coli and K. pneumoniae. |
| Keywords: | CMY-6 AmpC beta-lactamase CTX-M-15 ESBL India Concomitant resistance Oxyimino-cephalosporins 7-Alpha-methoxy-cephalosporins |
| 本文献已被 SpringerLink 等数据库收录！ | |