Silk fibroin has a protective effect against high glucose induced apoptosis in HIT-T15 cells |
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Authors: | Park Jun Hong Nam YoonYi Park So-Young Kim Jin-Kyung Choe Nong-Hoon Lee Jae-Young Oh Yang-Seok Suh Jun Gyo |
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Institution: | Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University, Gangwon-do 200-702, Republic of Korea. |
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Abstract: | High glucose levels induce cell death in many cell types, including pancreatic β-cells. Although protective agents against glucotoxicity have been searched for extensively, so far none have been found. In this report, we tested silk fibroin (SF) as a candidate material for antiglucotoxicity in the pancreatic β-cell (HIT-T15 cell) line. Approximately 50% of cells were killed after treatment with 80 mg/mL glucose. This reduction of cell number was recovered by the addition of SF at 50 mg/mL. SF treatment also decreased cellular reactive oxygen species (ROS) and increased proliferating cellular nuclear antigen (PCNA) immunoreactivity. In addition, TUNEL assays demonstrated that SF protects against glucose-induced apoptosis of HIT-T15 cells, suggesting that SF might protect cells from cell death by lowering cellular ROS levels. SF also induced expression of the insulin-like growth factor-1 (IGF-1) gene, and IGF-1 expression may be the cause of SF-induced protection against glucose toxicity. Taken together, these results suggest that SF could serve as a potential therapeutic agent to treat the hyperglycemia-induced death of pancreatic β-cells. |
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Keywords: | Silk Fibroin Glucotoxicity Apoptosis Reactive Oxygen Species Proliferating Cellular Nuclear Antigen Insulin‐Like Growth Factor‐1 |
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