Effect of inclusion bodies on the buoyant density of recombinant Escherichia coli |
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| Authors: | Soon Ook Hwang |
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| Affiliation: | (1) Dept. of Chemical Engineering, Tufts University, 02155 Medford, MA, USA;(2) Present address: Biotechnology Laboratory, Taedok Institute of Technology, Yukong Ltd., Taedok Science Town, 305-370 Taejon, Korea |
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| Abstract: | ![]()
Summary During batch culture, buoyant density of recombinant E. coli cells increased linearly as inclusion body per cell increased. This indicated that buoyant density can be used to follow inclusion body formation. This will be helpful to optimize product formation because inclusion bodies are mostly composed of foreign poteins produced by recombinant microorganisms. |
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