Phytochrome-mediated responses of cells and protoplasts of green calli obtained from the leaves of a CAM plant |
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Authors: | A Mricha J Brulfert J N Pierre O Queiroz |
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Institution: | (1) Institut de Physiologie Végétale, CNRS, F-91198 Gif-sur-Yvette Cedex, France |
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Abstract: | Green callus obtained from leaves of the CAM-inducible plant Kalanchoe blossfeldiana cv. Montezuma has previously been shown to perform C3-type photosynthesis under 16-h days and to shift to crassulacean acid metabolism (CAM) under 9-h days. The utilization of photoperiodic regimes (i.e. night interruptions by 30 min red light) established that CAM induction in the callus was under the control of phytochrome, as shown by measurements of CAM criteria: phosphoenolpyruvate carboxylase activity and malic acid pools. Short-term responsiveness of the callus cells to phytochrome modulations by monochromatic radiations was also established by the rapid changes observed in the diameter of the callus-derived protoplasts. These results provide further evidence that whole plant correlations are not necessary for phytochrome operativity.Abbreviations CAM
crassulacean acid metabolism
- PAL
phenylalanine ammonia lyase (EC 4.3.1.5)
- PAR
photosynthetically active radiations
- PEPC
phosphoenolpyruvate carboxylase (EC 4.1.1. 31)
- Rubisco
ribulose 1,5 bisphosphate carboxylase (EC 4.1.1.39) |
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