| 离子注入麦角甾醇酵母选育及发酵工艺 |
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| 引用本文： | 王纪,薛小莉. 离子注入麦角甾醇酵母选育及发酵工艺[J]. 微生物学杂志, 1998, 18(4): 25-28,38 |
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| 作者姓名： | 王纪 薛小莉 |
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| 作者单位： | 中国科学院等离子体物理研究所离子束生物工程研究室!合肥，230031，中国科学院等离子体物理研究所离子束生物工程研究室!合肥，230031，中国科学院等离子体物理研究所离子束生物工程研究室!合肥，230031，江西赣南制药厂，江西赣南制药厂，江西赣南制药厂 |
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| 基金项目： | “九五”国家重点攻关项目 |
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| 摘 要： | 通过离子注入诱变筛选到产麦角甾醇酵母高产菌，得率较出发菌提高55％～60％。对高产菌进行发酵条件优化，发现其对发酵通氧量有更高的要求，培养基中添加10％的糖或0．1％的Ca（NO3）2对麦角甾醇发酵有明显促进作用。
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| 关 键 词： | 离子注入 麦角甾醇 酿酒酵母 |
MUTATION BREEDING OF ERGOSTEROL-PRODUCING YEAST STRAIN BY IONS INJECTION AND ITS FERMENTATION TECHNOLOGY |
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| Wang Ji,et al.. MUTATION BREEDING OF ERGOSTEROL-PRODUCING YEAST STRAIN BY IONS INJECTION AND ITS FERMENTATION TECHNOLOGY[J]. Journal of Microbiology, 1998, 18(4): 25-28,38 |
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| Authors: | Wang Ji et al. |
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| Affiliation: | Wang Ji, et al. |
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| Abstract: | Mutants of ergbetero - preducing yeast strain were obtained by ion injection. The purity of ergostero pnduced by the mutants increased by 55 - 60% as compared with the original strain. The optiwhzation study on fermentation conditions for the high - yield strain indicated that the aeration needs a higher reqnirement. When 1.0% sugar and/or 0. 1% of Ca(NQ3)2 was added in the medium, the ergostero fermentation was rernarkably promoted. |
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| Keywords: | ion injection ergosterol Saccharomyces cerevisiae |
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