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Isolation of a novel stable peptide from cultivated Raphanus sativus with peroxidase activity |
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| Authors: | Omumi A. Morshedi D. Samadi A. |
| Affiliation: | (1) Department of Biochemistry, Pasteur Institute of Iran, Tehran, Pasteur St, 13164, Iran;(2) National Research Center for Genetic Engineering and Biotechnology, Tehran, P.O. Box 14155-6343, Iran;(3) Department of Biochemistry, Kirksville College of Osteopathic Medicine, 800 W, Jefferson St. Kirksville, MO 63501, USA |
| Abstract: | Isolation of a novel stable peptide from cultivated Raphanus sativus with peroxidase activity has been achieved using zinc ion precipitation followed by aqueous two phase extraction with a polyethylene glycol (PEG)/phosphate system. The best result was achieved with PEG 600/phosphate (28.8/13% w/v) which extracted 96.15% of total enzyme activity. The peptide has a molecular weight of 5.85 kD as determined by HPLC size exclusion method with an optimized pH of 6. |
| Keywords: | Aqueous two phase partitioning peroxidase Raphanus sativus |
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