Biodegradation of Carbofuran phenol by free and immobilized cells of <Emphasis Type="Italic">Klebsiella pneumoniae</Emphasis> ATCC13883T |
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Authors: | Jagannath C Kadakol Chandrappa M Kamanavalli Yogesh Shouche |
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Institution: | (1) Department of Chemistry, Karnatak Science College, 580 001 Dharwad, Karnataka, India;(2) National Center for Cell Science, Pune, India; |
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Abstract: | The Klebsiella sp. strain ATCC13883T capable of degrading carbofuran phenol (2,3-dihydro-2,2-dimethylbenzofuran-7-ol) has been separated
from the soil by enrichment culture technique and immobilized in various, namely polyurethane foam (PUF), polyacrylamide,
alginate, agar and alginate-bentonite clay-powdered activated charcoal (PAC). The degradation rates of 20 and 30 mM carbofuran
phenol by free and immobilized cells in batch and semi-continuous shaken cultures were compared. The PUF-immobilized cells
achieved higher degradation rates in a shorter time than freely suspended cells and the cells immobilized in polyacrylamide,
alginate and agar. The PUF- and alginate-bentonite clay-PAC-immobilized cells could be reused for more than 36 cycles, polyacrylamide-entrapped
cells for 20 cycles and alginate-bentonite-PAC 28 cycles, without losing any degradation capacity and showed better tolerance
to pH, temperature and concentration changes than free cells. These results showed that cells immobilized in modified alginate-bentonite-PAC
immobilizers tolerated and completely degraded carbofuran phenol at initial concentrations of 20 and 30 mM and also higher.
Such a bacterial strain could be used for bioremediation of environments contaminated with phenolic compounds. |
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