Evaluation of Sellers medium in identification of some gram-negative bacteria

The agar-slant medium of Sellers was evaluated as an identification aid in the differentiation between gram-negative bacilli of medical interest (Enterobacteriaceae excluded) and some of their saprophytic associates. With Sellers medium, a larger number of bacterial species and genera could be differentiated than with the oxidation-fermentation medium of Hugh and Leifson. Some species whose reclassification was recently suggested reacted on Sellers medium accordingly. Sellers medium (in a single tube) was found to be a distinct aid in the identification of a number of gram-negative bacteria which usually required several tests and an assortment of media.