Measurement of fatty acid oxidation in isolated perfused rat heart. A simple and accurate method |
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| Authors: | R.D. Evans X. Wang |
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| Affiliation: | 1. Nuffield Department of Anaesthetics, University of Oxford, Radcliffe Infirmary, Woodstock Road, Oxford, OX2 6HE, UK
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| Abstract: | Fatty acid oxidation is usually measured by collecting CO from [C]-labelled lipid. An alternative technique is to estimate HO production from [H]-lipid substrate; this has been used in working rat heart with [H]fatty acid and [H]triacylglycerol. HO appearance was linear and rates of [H]oleate and [H]triolein oxidation similar to [C]palmitate and [C]tripalmitin oxidation. Measurement of [H]lipid oxidation by HO estimation is simple, accurate, and a practicable alternative to the CO technique. |
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