Separation of estrogen conjugates by high pressure liquid chromatography. |
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| Authors: | P I Musey D C Collins J R Preedy |
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| Affiliation: | Departments of Medicine and Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30303 USA |
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| Abstract: | High pressure liquid chromatography using a prepacked commercial strong anion exchanger column (mu Partisil 10 SAX, 25 cm x 4.6 mm) was used to separate a mixture of eight estrogen conjugates. Chromatographic conditions using a 0.01 M potassium phosphate or 0.1 M NaCl as solvent in the isocratic mode are described for the separation of estrone glucosiduronate, 17beta-estradiol-3-glucosiduronate, 17beta-estradiol-17-glucosiduronate, estriol-3-glucosiduronate, estriol-16alpha-glucosiduronate, estriol-17-glucosiduronate, estrone sulfate and 17beta-estradiol-3-sulfate. This system gives high resolution of the estrogen conjugates in small eluent volumes in less than 30 min. The advantages of this high pressure liquid chromatographic system over other methods of separation are discussed. |
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| Keywords: | Mail reprint requests to: Dr. P. I. Musey Department of Medicine Emory University School of Medicine 69 Butler St. S. E- Atlanta Georgia 30303. |
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