Molecular cloning of grammistins, peptide toxins from the soapfish Pogonoperca punctata, by hemolytic screening of a cDNA library |
| |
Authors: | Kaji Tatsuyuki Sugiyama Nami Ishizaki Shoichiro Nagashima Yuji Shiomi Kazuo |
| |
Institution: | aDepartment of Food Science and Technology, Tokyo University of Marine Science and Technology, Konan-4, Minato-ku, Tokyo 108-8477, Japan |
| |
Abstract: | A novel method, based on the hemolytic screening of a cDNA phage library, was developed to isolate cDNAs encoding grammistins (antibacterial peptide toxins) of the soapfish Pogonoperca punctata. As a result, cDNAs encoding six grammistins were isolated and elucidated for their nucleotide sequences. In common with the grammistins, the precursor protein is composed of a highly conserved signal peptide, a considerably conserved propeptide that is characterized to contain a pair of basic residues (Lys-Arg) at plural positions including the C-terminus and one copy of a mature peptide. This precursor organization is similar to those of dermaseptins, antibacterial peptides from the frog skin. |
| |
Keywords: | cDNA cloning Grammistin Hemolytic screening Pogonoperca punctata Soapfish |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|