Role of stromal tenascin-C in mouse prostatic development and epithelial cell differentiation |
| |
Authors: | Ishii Kenichiro Imanaka-Yoshida Kyoko Yoshida Toshimichi Sugimura Yoshiki |
| |
Institution: | a Department of Nephro-Urologic Surgery and Andrology, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507, Japan b Department of Pathology and Matrix Biology, Mie University Graduate School of Medicine, Japan |
| |
Abstract: | Deregulation of epithelial-stromal interactions is considered to play a critical role in the initiation and promotion of benign prostatic hyperplasia (BPH) and prostate carcinoma (PCa). Expression of tenascin-C (TN-C), an extracellular matrix (ECM) glycoprotein, is reportedly higher in BPH and PCa as compared with normal prostate. Remodeling of the ECM alters the homeostatic balance between epithelium and stroma, resulting in physiological changes in cellular functions. To investigate the role of TN-C in prostatic development and differentiation, we evaluated the morphological phenotype of TN-C knockout (KO) mouse prostate (ventral: VP, dorsolateral: DLP, and anterior: AP) and examined tissue recombinants composed of adult mouse DLP epithelium and fetal TN-C KO urogenital sinus mesenchyme (UGM). Histological analysis showed epithelial cell clusters protruding into the ductal lumens in TN-C KO AP and DLP. Interestingly, binucleated cells appeared in epithelium of TN-C KO DLP at 8 weeks. Simultaneously, androgen receptor (AR)-positive cells were decreased in TN-C KO epithelia. Similar to the TN-C KO phenotype, protruded epithelial clusters, binucleated cells, and AR-negative nuclei were induced in DLP epithelium by recombining with TN-C KO UGM. Our results suggest that stromal TN-C might be involved in maintaining epithelial cytodifferentiation, morphogenesis, and androgen receptor expression of normal prostate glands in adult mice. |
| |
Keywords: | Tenascin-C Knockout mouse Prostate Epithelial-stromal interactions Epithelial cell differentiation Epithelial cell cluster Binucleated cell Androgen receptor Tissue recombination Urogenital sinus mesenchyme |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|