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Variation of wheatgrass chromosomes in wheat-wheatgrass alien addition line "TAI-27"revealed by fluorescence in situ hybridization (FISH)
Authors:HAN Fangpu  ZHANG Xiangqi  BU Xiuling  HE Mengyuan  HAO Shui
Abstract:
The karyotype of the primary wheat wheatgrass alien addition line TAI 27 was 2 n =44 in which all of the chromosomes were metacentric and submetacentric. However, in the progeny of TAI 27 a pair of chromosomes had become small chromosomes in the two morphologically different plants. Fluorescence in situ hybridization (FISH)technique was used to analyze the two different plants. The observations indicate that a pair of small chromosomes in one variation line are from wheatgrass. In another variation line, a pair of small chromosomes are also from wheatgrass, while another pair of wheatgrass chromosomes have substituted the wheat chromosomes. TAI 27 and its variant lines showed a high level of resistance to barley yellow dwarf virus (BYDV). The possible explanation for such a variation and the potential use of the variant lines were discussed briefly.
Keywords:wheat   wheatgrass   alien addition line   alien addition substitution line   fluorescence in situ {hybridi|}
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