Abstract: | ![]() The karyotype of the primary wheat wheatgrass alien addition line TAI 27 was 2 n =44 in which all of the chromosomes were metacentric and submetacentric. However, in the progeny of TAI 27 a pair of chromosomes had become small chromosomes in the two morphologically different plants. Fluorescence in situ hybridization (FISH)technique was used to analyze the two different plants. The observations indicate that a pair of small chromosomes in one variation line are from wheatgrass. In another variation line, a pair of small chromosomes are also from wheatgrass, while another pair of wheatgrass chromosomes have substituted the wheat chromosomes. TAI 27 and its variant lines showed a high level of resistance to barley yellow dwarf virus (BYDV). The possible explanation for such a variation and the potential use of the variant lines were discussed briefly. |