Isolation of DNA from plants high in polyphenolics |
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| Authors: | Jennifer A. Couch Paul J. Fritz |
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| Affiliation: | (1) ACRI Cocoa Molecular Biology Lab, The Intercollege Program of Genetics, Pennsylvania State University, 16802 University Park, PA, USA;(2) The Department of Food Science, Pennsylvania State University, 16802 University Park, PA, USA |
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| Abstract: | DNA suitable for digestion with restriction endonucleases has been isolated fromTheobroma cacao, a plant high in polyphenolic compounds, and several other plant taxa using the method presented in this paper. The method
relies on concentrating nuclei away from most of the cytoplasmic components and inhibiting the formation of oxidized polyphenolic
compounds and their interaction with the DNA fraction in the remaining steps. |
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| Keywords: | DNA isolation polyphenols
Theobroma cacao |
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