抗氧化肽SS-31抑制高糖诱导的心肌细胞凋亡

该文探讨了抗氧化肽SS-31对高糖诱导的大鼠心肌细胞凋亡的影响,体外培养大鼠心肌细胞(H9C2),给予高糖(30 mmol/L)进行刺激,并利用抗氧化肽SS-31进行治疗后,采用Western blot检测Bax、Bcl-2、Cleaved caspase-3、Nox1、Nox4和Txnip的表达;利用荧光实时定量PCR(Real-time PCR)检测Bax、Bcl-2、Nox1、Nox4和Txnip mRNA的表达;采用原位缺口末端标记法(TUNEL)观察H9C2细胞凋亡情况;应用MitoSOXTM Red染色检测H9C2细胞线粒体ROS产生情况。结果显示,与正常对照组相比,高糖组H9C2细胞凋亡明显增加,Bax、Cleaved caspase-3、Nox1、Nox4和Txnip蛋白表达明显上调,Bcl-2蛋白表达减少,线粒体ROS产生增加;SS-31治疗后能够抑制高糖诱导的H9C2细胞的凋亡,下调Bax、Cleaved caspase-3、Nox1、Nox4、Txnip表达,上调Bcl-2表达并减少线粒体ROS的产生。以上研究结果提示,抗氧化肽SS-31抑制高糖诱导的大鼠心肌细胞凋亡的同时抑制Txnip/ROS产生,表明抗氧化肽SS-31可能对糖尿病心肌病变具有一定的改善作用。

Antioxidant Peptide SS-31 Inhibits High Glucose Induced Cardiomyocyte Apoptosis

The aim of this article was to investigate the effect of antioxidant peptide SS-31 on HG(high glucose)-induced apoptosis in rat cardiomyocytes.In vitro culture of rat cardiomyocytes(H9C2)were stimulated with high glucose(30 mmol/L),and treated with antioxidant peptide SS-31.The protein expressions of Bax,Bcl-2,Cleaved caspase-3,Nox1,Nox4 and Txnip were observed by Western blot.The mRNA expressions of Bax,Bcl-2,Nox1,Nox4 and Txnip were observed by Real-time PCR.The nick end labeling method(TUNEL)was used to observe the apoptotic H9C2 cells situation.MitoSOXTM Red staining was used to detect the production of mitochondrial ROS in H9C2 cells.Compared with the normal control group,the apoptosis of H9C2 cells,was significantly increased.The proteins expression of Bax,Cleaved caspase-3,Nox1,Nox4 and Txnip was up-regulated.The expression of Bcl-2 protein was decreased,and the production of mitochondrial ROS was increased in high glucose stimulated H9C2 cells.Treatment with SS-31 could inhibit the apoptosis of H9C2 cells,down-regulating the expression of Bax,Cleaved caspase-3,Nox1,Nox4 and Txnip,up-regulating the expression of Bcl-2 and reduce the production of mitochondrial ROS in high glucose induced H9C2 cells.Above mentioned results suggest that SS-31 inhibits HG-induced rat cardiomyocytes apoptosis and TXNIP/ROS production,indicating that antioxidant peptide SS-31 may have a certain improvement effect on diabetic cardiomyopathy.