| 氧化葡萄糖酸杆菌SCB329染色体的测定 |
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| 引用本文： | 林文楚,尹光琳.氧化葡萄糖酸杆菌SCB329染色体的测定[J].微生物学报,2001,41(1):40-53. |
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| 作者姓名： | 林文楚 尹光琳 |
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| 作者单位： | 中国科学院上海生物工程研究中心,上海,200233 |
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| 基金项目： | 国家自然科学基金资助项目(39970024),获得河北维生药业公司科研合作经费资助 |
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| 摘 要： | 对氧化葡萄糖酸杆菌(\%Gluconobacter oxydans\%)SCB329的纯培养进行了研究，测定了其生长曲线，确定其对数期为4～27h。获得纯培养的对数期菌体后采用凝胶包埋法制备完整染色体，用脉冲场电泳方法对SCB329的染色体进行了分析，确定其有一条染色体和一个大质粒。染色体的长度在22Mb到35Mb之间。
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| 关 键 词： | 氧化葡萄糖酸杆菌， 纯培养， 凝胶包埋法，脉冲电泳，染色体 |
| 文章编号： | 0001-6209(2001)01-0049-05 |
DETERMINATION OF CHROMOSOME OF GLUCONOBACTER OXYDANS SCB329 |
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| W Lin,G Yin.DETERMINATION OF CHROMOSOME OF GLUCONOBACTER OXYDANS SCB329[J].Acta Microbiologica Sinica,2001,41(1):40-53. |
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| Authors: | W Lin G Yin |
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| Institution: | Shanghai Research Centre of Biotechnology, Chinese Academy of Sciences, Shanghai 200233, China. |
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| Abstract: | After the pure culture of Gluconobacter oxydans SCB329 was researched, its growth curve was measured and its logarithmic phase was determined as between 4-24 h. After the microorganisms were havested in its logarithmic phase, The intact chromosome was prepared by agarose-embedded method. Then the genome of SCB329 was analyzed by Pulsed-field Gel Electrophoresis. The result indicated that there are one chromosome and one great plasmid. The length of intact chromosome of SCB329 has been estimated to be approximately between 2.2 Mb and 3.5 Mb. |
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| Keywords: | Gluconobacter oxydans Pure Culture Agarose\|embedded method Pulsed\|field gel electrophoresis Chromosome |
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