Somatic embryogenesis and plant regeneration in centipedegrass (Eremochloa ophiuroides [Munro] Hack.) |
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Authors: | Mingxi Liu Jing Yang Shaoyun Lu Zhenfei Guo Xiping Lin Hong Wu |
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Institution: | (1) Biotechnology Laboratory for Turfgrass and Forages, College of Life Science, South China Agricultural University, Guangzhou, 510642, People’s Republic of China |
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Abstract: | Centipedegrass (Eremochloa ophiuroides Munro] Hack.) is an important warm-season turfgrass and pasture grass. To explore the potential use of biotechnical tools
in breeding of centipedegrass, we established an efficient plant regeneration system for this species. Four basal media and
24 combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzyladenine (BAP) were examined for their effects on callus
induction from mature seed explants. Twenty combinations of naphthaleneacetic acid (NAA) and BAP were tested for their effect
on plant regeneration. Results indicated that Murashige and Skoog basal medium supplemented with 4.5 mg l−1 2,4-D and 1 mg l−1 BAP was the best medium for callus induction, while the combination of 2 mg l−1 BAP and 1 mg l−1 NAA induced the highest rate of regeneration and development of shoots and roots. This work provides a basis for the breeding
of centipedegrass through somaclonal variation and genetic transformation. |
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Keywords: | Centipedegrass Eremochloa ophiuroides Somatic embryogenesis Plant regeneration |
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