| 白萝卜提取物诱生干扰素的有效成份 |
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| 引用本文： | 王燕平,许兆祥,曲凤珍,童伶莉.白萝卜提取物诱生干扰素的有效成份[J].病毒学报,1988(3). |
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| 作者姓名： | 王燕平 许兆祥 曲凤珍 童伶莉 |
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| 作者单位： | 中国预防医学科学院病毒学研究所 北京
(王燕平，许兆祥，曲凤珍)，中国预防医学科学院病毒学研究所 北京(童伶莉) |
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| 摘 要： | 本实验用CF-11纤维素柱层析,从白萝卜提取物中分离纯化到一种双链RNA,简称E.R.s.r-dsRNA(全称见正文)。实验证明,E.R.s.r-dsRNA在家兔体内可诱生高水平的干扰素;纳克(ng)水平即可在体外细胞培养中抑制病毒感染。E.R.s.r-dsRNA分子量略小于1.34×10~6道尔顿,它对RNase的耐受性比Poly Ⅰ:PolyC强。推测E.R.s.r-dsRNA可能有较好的应用前景。
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| 关 键 词： | 白萝卜提取物 双链RNA 干扰素诱生剂 |
STUDIES ON THE ACTIVE PRINCIPLE INDUCING INTERFERON IN THE EXTRACTOF RAPHANUS SATIVUS-ROOT |
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| Wang Yanping Xu Zhaoxiang Qu Fengzhen Tong Lingli.STUDIES ON THE ACTIVE PRINCIPLE INDUCING INTERFERON IN THE EXTRACTOF RAPHANUS SATIVUS-ROOT[J].Chinese Journal of Virology,1988(3). |
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| Authors: | Wang Yanping Xu Zhaoxiang Qu Fengzhen Tong Lingli |
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| Abstract: | Using cellulose (CF-11 ) chromatography a double stranded RNA was purified from the extract of R.sativus-root, termed E.R.s.r-dsRNA. E.R. s.r-dsRNA is capable of inducing a high level of interferon in rabbit and is dctive at nanogram level in inducing resistance to virai infection in vitro. E.R.s.r-dsRNA is about 2.0kb in length and is relatively resistant to RNase as compared with synthetic Poly I:Poly C. Based on the results ob-tained by us it is reasonable to speculate that E.R.s.r-dsRNA may have a good prospect for pratical use. |
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| Keywords: | Extract of Raphantts sativus root(E R s r ) Double-stranded RNA Interferon inducer |
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