Lipidomic analysis reveals prostanoid profiles in human term pregnant myometrium |
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| Authors: | J.H. Durn K.M. Marshall D. Farrar P. O’Donovan A.J. Scally D.F. Woodward A. Nicolaou |
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| Affiliation: | 1. School of Pharmacy, University of Bradford, Bradford BD7 1DP, UK;2. Bradford Royal Infirmary, Bradford BD9 6RJ, UK;3. School of Health Studies, University of Bradford, Bradford BD5 0BB, UK;4. Allergan Inc., Irvine, CA 9612, USA;1. Department of Chemistry, Washington State University, Pullman, WA, USA;2. Department of Cell and Developmental Biology and Program in Structural Biology and Biophysics, University of Colorado, Health Sciences Center, Anschutz Medical Campus, Aurora, CO, USA;1. University of Paris-Descartes, Faculty of Pharmacy, Laboratory of Physical Chemistry, 4 avenue de l’Observatoire, 75006 Paris, France;2. University of Paris-Sud, Faculty of Pharmacy, Groupe Matériaux et Santé EA 401, 5 rue Jean-Baptiste Clément, 92296 Châtenay-Malabry, France;3. Assistance Publique-Hôpitaux de Paris, Agence Générale des Equipements et Produits de Santé, Département de Contrôle Qualité et Développement Analytique, 7 rue du Fer à Moulin, 75005 Paris, France;4. Solvay SA, 25 rue de Clichy, 75009 Paris, France;5. 78170 La Celle St Cloud, France;6. University of Monastir, Faculty of Pharmacy, Laboratory of Analytical Chemistry, rue Ibn Sian, 5000 Monastir, Tunisia;1. School of Aquatic and Fishery Sciences, Box 355020, University of Washington, Seattle, WA 98195, United States;2. Tern Again Consulting, 388 E. Bayview Ave., Homer, AK, United States;3. U.S. Fish and Wildlife Service, 1011 E. Tudor Rd., Anchorage, AK 99503, United States;1. Department of Chemistry, Washington State University, Pullman, WA 99164, USA;2. Pfizer Global Research & Development, Groton Laboratories, Pfizer Inc., Groton, CT 06340, USA;3. Structural Chemistry Group (SCG), Irvine Pharmaceutical Services, Irvine, CA 92618, USA;1. Department of Gynecology and Gynecologic Oncology, Medical University of Bialystok, 24a Sklodowskiej-Curie Str., 15-269 Bialystok, Poland;2. Department of Physiology, Medical University of Bialystok, 2c Mickiewicza Str., 15-222 Bialystok, Poland;3. Department of Perinatology, Medical University of Bialystok, 24a Sklodowskiej-Curie Str., 15-269 Bialystok, Poland |
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| Abstract: | ![]()
Prostanoids modulate the activity of human pregnant myometrium and their functional role can be appreciated through characterisation of prostanoid receptors and tissue concentration of prostanoids. We have applied a lipidomic approach to elucidate the profile of prostanoids in human non-labouring and labouring myometrium. We have identified a total of nineteen prostanoids including prostacyclin, thromboxanes, prostaglandins and dihydro-prostaglandins. Prostacyclin was the predominant prostanoid in both non-labouring and labouring myometria, with PGD2 and PGF2α being the second most abundant. Although the total amount of prostanoids was increased in the labouring tissue, PGE2 and 13,14-dihydro-15-keto-PGE2 were the only prostanoids to increase significantly at early and late labour (p≤0.001). Our data suggest that PGF2α plays an important role in parturition, whilst the increase in PGE2 could occur to facilitate cervical dilation and relaxation of the lower myometrium during labour. Although the elevation in TXA2 was less marked than expected, in terms of translation to function even a relatively small increase in the level of this potent spasmogen may have significant effects. |
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