Novel recombinant human lactoferrin: Differential activation of oxidative stress related gene expression |
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| Authors: | Marian L. Kruzel Jeffrey K. Actor Michał Zimecki Jasen Wise Paulina Płoszaj Shaper Mirza Mark Kruzel Shen-An Hwang Xueqing Ba Istvan Boldogh |
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| Affiliation: | 1. Department of Integrative Biology and Pharmacology, University of Texas-Houston, United States;2. Department of Pathology and Laboratory Medicine, University of Texas-Houston, United States;3. School of Public Health, University of Texas-Houston, United States;4. Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroc?aw, Poland;5. QIAGEN, Frederick, MD, United States;6. Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, TX, United States;g Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China;h Wroc?aw Medical University, Wroc?aw, Poland |
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| Abstract: | Lactoferrin, an iron-binding protein found in high concentrations in mammalian exocrine secretions, is an important component of the host defense system. It is also a major protein of the secondary granules of neutrophils from which is released upon activation. Due to its potential clinical utility, recombinant human lactoferrin (rhLF) has been produced in various eukaryotic expression systems; however, none of these are fully compatible with humans. Most of the biopharmaceuticals approved by the FDA for use in humans are produced in mammalian expression systems. The Chinese hamster ovary cells (CHO) have become the system of choice for proteins that require post-translational modifications, such as glycoproteins. |
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| Keywords: | hLF, human milk lactoferrin rhLF, recombinant human lactoferrin TF, transferrin LPS, lipopolysaccharide HEK, human epithelial kindney cells CHO, Chinese hamster ovary cells ELISA, enzyme-linked immunosorbent assay CFU, colony forming units MTX, methotrexate BCG, Bacillus Calmette&ndash Gué rin vaccine ROS, reactive oxygen species SOD, superoxide dismutase GPX, glutathione peroxidase PRDX, peroxiredoxins PTGS, prostaglandin-endoperoxide synthase COX, cyclooxygenase MALDI-TOF, matrix-assisted laser desorption/ionization time of flight ICP-MS, inductively coupled plasma mass spectrometry |
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