Interconversion of ketoprofen recognition in firefly luciferase-catalyzed enantioselective thioesterification reaction using from Pylocoeria miyako (PmL) and Hotaria parvura (HpL) just by mutating two amino acid residues |
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Authors: | Dai-ichiro Kato Yoshihiro Hiraishi Mika Maenaka Keisuke Yokoyama Kazuki Niwa Yoshihiro Ohmiya Masahiro Takeo Seiji Negoro |
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Institution: | 1. Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Shosha, Himeji, Hyogo, 671-2280, Japan;2. National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, 305-8563, Japan |
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Abstract: | We identified the critical amino acid residues for substrate recognition using two firefly luciferases from Pylocoeria miyako (PmL) and Hotaria parvura (HpL), as these two luciferase enzymes exhibit different activities toward ketoprofen. Specifically, PmL can catalyze the apparent enantioselective thioesterification reaction, while HpL cannot. By comparing the amino acid sequences around the active site, we identified two residues (I350 and M397 in PmL and F351 and S398 in HpL) that were different between the two enzymes, and the replacement of these amino acids resulted in changing the ketoprofen recognition pattern. The inactive HpL was converted to the active enzyme toward ketoprofen and vice versa for PmL. These residues also affected the enantioselectivity toward ketoprofen; however, the bioluminescent color was not affected. In addition, using molecular dynamics calculations, the replacement of these two amino acids induced changes in the state of hydrogen bonding between ketoprofen and the S349 side chain through the active site water. As S349 is not considered to influence color tuning, these changes specifically caused the differences in ketoprofen recognition in the enzyme. |
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Keywords: | PmL firefly luciferase from Pylocoeria miyako HpL firefly luciferase from Hotaria parvura LUC-H firefly luciferase from Luciola lateralis PpL firefly luciferase from Photinus pyralis LcL firefly luciferase from Luciola cruciate LmL firefly luciferase from Luciola mingrelica MD molecular dynamics CoASH coenzyme A PPB potassium phosphate buffer |
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