Daily variation in circulating lymphocyte counts, T and B proportions and responsiveness to phytohemagglutinin. |
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| Authors: | P T Fan D T Yu P J Clements G Opelz L Goldberg R Bluestone |
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| Institution: | 1. From the Medical and Research Services, VA Wadsworth Hospital Center, Wilshire and Sawtelle Blvds., Los Angeles, California 90073, USA;2. Department of Me Medicine, UCLA School of Medicine, Los Angeles, California 90024, USA |
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| Abstract: | Peripheral blood was obtained from six healthy individuals over five consecutive days under uniform conditions and the total lymphocyte counts, T and B proportions, and response to phythohemagglutinin (PHA) were determined. The daily variation in T lymphocytes as measured by the spontaneous sheep erythrocyte (SRBC) assay was much greater when total T concentrations rather than T percentages were compared. There was considerable daily variation in PHA responsiveness and in the percentages of cells bearing Fc and C3 receptors and surface immunoglobulin (SIg). Cryopreservation did not affect the proportions of T and B lymphocytes although it resulted in a significant enhancement of PHA responsiveness following the freeze-thaw procedure. The significance of these results is discussed. |
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