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Introduction of a nitrogen-fixing cyanobacterium into tobacco shoot regenerates
Authors:M. V. Gusev  T. G. Korzhenevskaya  L. V. Pyvovarova  O. I. Baulina  R. G. Butenko
Affiliation:(1) Department of Cell Physiology and Immunology, Faculty of Biology, Moscow State University, 119899 Moscow, GSP;(2) Institute of Plant Physiology, Botanicheskaya, 35, 127276 Moscow, USSR
Abstract:
Tobacco (Nicotiana tabacum L.) shoots associated with the nitrogen-fixing cyanobacterium Anabaena variabilis Kütz. (ATCC 29413) were regenerated in mixed cultures of tobacco callus and the cyanobacterium. The cyanobacteria were localized inside the tissues as well as on the surface of regenerated shoots, formed heterocysts, and were capable of acetylene reduction.
Keywords:Acetylene reduction  Anabaena  Callus culture  Cyanobacteria  Nicotiana (association with cyanobacterium)  Nitrogenase activity  Regeneration in tissue culture
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