A method for the ligation of DNA following isolation from low melting temperature agarose |
| |
Authors: | D M Burns I R Beacham |
| |
Institution: | School of Science, Griffith University, Nathan, Brisbane, Queensland 4111, Australia |
| |
Abstract: | A rapid, simple, and reliable method is presented for the isolation and subsequent ligation of DNA from agarose gels. The technique involves the use of low melting temperature agarose, but with the inclusion of bovine serum albumin or gelatin to the ligation reaction. |
| |
Keywords: | DNA ligation agarose electrophoresis |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|