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淫羊藿总黄酮的超声辅助提取及其抗氧化活性
引用本文:张俊生,陈莉华,汤小虎,张烨,李三艳.淫羊藿总黄酮的超声辅助提取及其抗氧化活性[J].生物加工过程,2012,10(1):13-18.
作者姓名:张俊生  陈莉华  汤小虎  张烨  李三艳
作者单位:吉首大学化学化工学院,吉首,416000
基金项目:科技部科技型中小企业技术创新基金资助项目,吉首大学校级科研计划项目
摘    要:利用超声波辅助乙醇提取淫羊藿中的总黄酮,通过单因素和正交试验法获得最佳工艺条件后,考察黄酮提取物对油脂的抗氧化性能及对·OH的清除效果.结果表明:体积分数70%的乙醇、料液比(g/mL) 1:30、超声提取时间30 min、超声温度50℃时为最佳条件,得到淫羊藿提取物中总黄酮质量分数为2.246%.淫羊藿总黄酮对·OH清除效果随浓度的增大而升高,对油脂氧化亦有较强的抑制作用.

关 键 词:抗氧化  淫羊藿  油脂  总黄酮

Ultrasonic-assisted ethanol extraction of flavonoids from Epimedium and their antioxidative effects on fats and oils
ZHANG Junsheng,CHEN Lihua,TANG Xiaohu,ZHANG Ye,LI Sanyan.Ultrasonic-assisted ethanol extraction of flavonoids from Epimedium and their antioxidative effects on fats and oils[J].Chinese Journal of Bioprocess Engineering,2012,10(1):13-18.
Authors:ZHANG Junsheng  CHEN Lihua  TANG Xiaohu  ZHANG Ye  LI Sanyan
Institution:(College of Chemistry and Chemical Engineering,Jishou University,Jishou 416000,China)
Abstract:Flavonoids were extracted from Epimedium with ultrasonic assistance,using ethanol as extraction solvent.The optimal extracting conditions were obtained based on single factor test and orthogonal experimental design.The hydroxyl radical-scavenging capacities and antioxidative activities against the peroxidation of oils and fats of the flavonoid extracts were obtained compared with reference antioxidants.Results showed that under the conditions of 70% ethanol,1∶30(g/mL) material-to-liquid ratio,and 30 min ultrasonic extracting time,the total flavonoid mass fraction of extracts was 2.246%.The extracts showed good antioxidative effects on oils and fats.
Keywords:antioxidation  Epimedium  fats and oils  flavonoids
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