Potential detoxification of gossypol by UDP-glycosyltransferases in the two Heliothine moth species Helicoverpa armigera and Heliothis virescens |
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| Institution: | 1. Department of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Str. 8, 07745 Jena, Germany;2. Department of Biochemistry, Max Planck Institute for Chemical Ecology, Hans-Knoell-Str. 8, 07745 Jena, Germany;1. School of Biology and Ecology, University of Maine, 5722 Deering Hall, Orono, ME 04469, United States;2. Department of Plant and Soil Science, 63 Carrigan Dr., University of Vermont, Burlington, VT 05405, United States;1. Department of Entomology and BIO5 Institute, University of Arizona, Tucson, AZ 85721, United States;2. School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, Henan, China;1. College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;2. Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej, Denmark;3. Department of Entomology, Max Planck Institute for Chemical Ecology, Jena 07745, Germany;1. Laboratory of Fundamental and Applied Research in Chemical Ecology, University of Neuchâtel, 2000 Neuchâtel, Switzerland;2. Neuchâtel Platform of Analytical Chemistry, University of Neuchâtel, 2000 Neuchâtel, Switzerland;3. Laboratory of Organic Chemistry, University of Neuchâtel, 2000 Neuchâtel, Switzerland;4. Max Planck Institute for Chemical Ecology, 07745 Jena, Germany;5. Laboratory of Phytochemistry and Bioactive Natural Products, University of Geneva, University of Lausanne, 1211 Geneva, Switzerland |
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| Abstract: | The cotton bollworm Helicoverpa armigera and the tobacco budworm Heliothis virescens are closely related generalist insect herbivores and serious pest species on a number of economically important crop plants including cotton. Even though cotton is well defended by its major defensive compound gossypol, a toxic sesquiterpene dimer, larvae of both species are capable of developing on cotton plants. In spite of severe damage larvae cause on cotton plants, little is known about gossypol detoxification mechanisms in cotton-feeding insects. Here, we detected three monoglycosylated and up to five diglycosylated gossypol isomers in the feces of H. armigera and H. virescens larvae fed on gossypol-supplemented diet. Candidate UDP-glycosyltransferase (UGT) genes of H. armigera were selected by microarray studies and in silico analyses and were functionally expressed in insect cells. In enzymatic assays, we show that UGT41B3 and UGT40D1 are capable of glycosylating gossypol mainly to the diglycosylated gossypol isomer 5 that is characteristic for H. armigera and is absent in H. virescens feces. In conclusion, our results demonstrate that gossypol is partially metabolized by UGTs via glycosylation, which might be a crucial step in gossypol detoxification in generalist herbivores utilizing cotton as host plant. |
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| Keywords: | Host plant adaptation Gossypol detoxification UDP-Glycosyltransferase DMSO"} {"#name":"keyword" "$":{"id":"kwrd0040"} "$$":[{"#name":"text" "_":"dimethyl sulfoxide P450"} {"#name":"keyword" "$":{"id":"kwrd0050"} "$$":[{"#name":"text" "_":"cytochrome P450 monooxygenase UGT"} {"#name":"keyword" "$":{"id":"kwrd0060"} "$$":[{"#name":"text" "_":"UDP-glycosyltransferase |
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