Purple carrot extract protects against cadmium intoxication in multiple organs of rats: Genotoxicity,oxidative stress and tissue morphology analyses |
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| Institution: | 1. Department of Biosciences, Federal University of Sao Paulo, UNIFESP, SP, Brazil;2. ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, University of Campinas (UNICAMP), Campinas, SP, Brazil;3. Department of Pathology, Paulista Medical School, Federal University of Sao Paulo, UNIFESP, SP, Brazil;1. Division of Thoracic Surgery, Swedish Cancer Institute, Seattle, Washington;2. Section of General Thoracic Surgery, Department of Surgery, UC Davis Medical Center, Sacramento, California;3. Unit of Thoracic Surgery, Catholic University “Sacred Heart,” Rome, Italy;4. Center of Thoracic Surgery, University of Insubria, Ospedale di Circolo, Varese, Italy;5. Division of Cardiothoracic Surgery, University of Washington Medical Center, Seattle, Washington;6. Division of Thoracic Surgery, Providence Regional Medical Center, Everett, Washington;7. Division of Thoracic Surgery, Virginia Mason Hospital and Seattle Medical Center, Seattle, Washington;1. Laboratory of Spectroscopy of Inorganic Compounds, Nikolaev Institute of Inorganic Chemistry SB RAS, 3, Acad. Lavrentiev Ave., Novosibirsk 630090, Russia;2. Faculty of Natural Sciences, Novosibirsk State University, 2 Pirogova Str., Novosibirsk 630090, Russia;3. Laboratory of Clinical Morphology, Institute of Molecular Pathology and Pathomorphology SB RAMS, 2 Timakova Str., Novosibirsk 630060, Russia;4. Laboratory of Functional Morphology of Lymphatic System, Scientific Institution of Clinical and Experimental Lymphology SB RAMS, 2 Timakova Str., Novosibirsk 630060, Russia;5. Laboratory 8-2, Budker Institute of Nuclear Physics SB RAS, 11, Acad. Lavrentiev Ave., Novosibirsk 630090, Russia;1. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, Henan, 471023, China;2. College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, Henan, 471023, China;1. College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, 225009, PR China;2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, 225009, PR China;3. Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, PR China |
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| Abstract: | The aim of this study was to investigate if purple carrot extract is able to protect against the noxious activities induced by cadmium exposure in multiple organs of rats. For this purpose, histopathological analysis, genotoxicity and oxidative status were investigated in this setting. A total of twenty Wistar rats weighing 250 g on the average, and 8 weeks age were distributed into four groups (n = 5), as follows: Control group (non-treated group, CTRL); Cadmium group (Cd) and Purple carrot extract groups at 400 mg/L or 800 mg/L. Histopathological analysis revealed that liver from animals treated with purple carrot extract improved tissue degeneration induced by cadmium intoxication. Genetic damage was reduced in blood and hepatocytes as depicted by comet and micronucleus assays in animals treated with purple carrot extract. SOD-CuZn and cytocrome C gene expression increased in groups treated with purple carrot extract. Purple carrot extract also reduced the 8OHdG levels in liver cells when compared to cadmium group. Taken together, our results demonstrate that purple carrot extract is able to protect against cadmium intoxication by means of reducing tissue regeneration, genotoxicity and oxidative stress in multiple organs of Wistar rats. |
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| Keywords: | Purple carrot Cadmium Rat Genotoxicity Oxidative stress |
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