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| 海南和两广地区柱花草炭疽菌遗传多态性的RAPD分析 | |
| 引用本文： | 史学群 宋海超 张欣 易克贤. 海南和两广地区柱花草炭疽菌遗传多态性的RAPD分析[J]. 植物学报, 2007, 24(2): 173-180 |
| 作者姓名： | 史学群 宋海超 张欣 易克贤 |
| 作者单位： | 1 海南大学生命科学与农学院, 海口 570228; 2 中国热带农业科学院环境与植物保护研究所, 儋州 571737 |
| 基金项目： | 国家自然科学基金,海南省自然科学基金,热带作物生物技术国家重点实验室开放研究基金 |
| 摘 要： | 利用8个随机引物对来自海南、广东和广西的114个柱花草胶孢炭疽菌(Colletotrichum gloeosporioides)的菌株进行了随机扩增多态性DNA(RAPD)分析, 扩增谱带大小0.2-3.0 kb。8个随机引物中以CW38114扩增的谱带重现性和稳定性最好, 共扩增出786条谱带, 其中多态性谱带558条。供试菌株扩增图谱在260-650 bp处有3条明显的共同特征谱带, 在650-3 000 bp之间的谱带显示较大的多态性差异。聚类结果表明: 供试菌株主要分为四大遗传类型, 其中第I类和第III类有两个亚类; 三省区的菌株遗传多态性丰富程度依次为海南>广西>广东, 并表现出地区性分布差异。研究结果还表明柱花草炭疽菌株表现出一定程度的专化寄生性。 |
| 关 键 词： | 胶孢炭疽菌 随机扩增多态性DNA 柱花草 |
| 收稿时间： | 2006-06-21 |
| 修稿时间： | 2006-11-20 |
Genetic Diversity Analysis of Colletotrichum gloeosporioides from Stylosanthes spp in Hainan, Guangdong and Guangxi with Use of Random-amplified Polymorphic DNA |
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| Xuequn Shi,Haichao Song,Xin Zhang,Kexian Yi. Genetic Diversity Analysis of Colletotrichum gloeosporioides from Stylosanthes spp in Hainan, Guangdong and Guangxi with Use of Random-amplified Polymorphic DNA[J]. Bulletin of Botany, 2007, 24(2): 173-180 | |
| Authors: | Xuequn Shi Haichao Song Xin Zhang Kexian Yi |
| Affiliation: | 1Life Sciences and Agriculture College, Hainan University, Haikou 570228, China;2Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China |
| Abstract: | Genetic diversity of 114 isolates of Stylosanthes spp of Colletotrichum gloeosporioides in Hainan, Gunagdong andGuangxi was analyzed by use of random amplified polymorphic DNA (RAPD) and 8 arbitrary 10-base oligonucleotide primers. Theamplified fragments were 0.2-3.0 kb. A total of 786 well-replicated, clear and stable bands were amplified by use of the primer CW38114. The results showed good DNA polymorphism between isolates. Four significant common bands were found between 260 and 650 bp in the amplification profile. However, between 650 and 3 000 bp showed large differences. The grouping analysis showed all the isolates grouped in four clusters, and clusters I and III could be divided into two subgroups each. The genetic variation differed by provinces: Hainan > Guangxi > Gunagdong. These isolates also showed parasitic specialization to some extent. |
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