The use of Taka-diastase in a[3H]poly(A) hybridization assay of oligo(U) sequences in RNA |
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| Authors: | E De Herdt M Kondo H Slegers |
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| Institution: | Department of Cell Biology, University of Antwerp, Universiteitsplein 1, B-2610 Wilrijk, Belgium |
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| Abstract: | A reliable assay of uridylate sequences longer than 10 is described. The procedure is based on the hybridization of 3H]poly(A) with poly(U) or oligo(U) sequences in high ionic conditions and a subsequent degradation of single stranded polynucleotides with purified Taka-diastase. A 1:2 complex between poly(A) and poly(U) is formed on which on poly(U) strand is digested by Taka-diastase. The procedure is especially suitable for the detection and quantitation of Un (n greater than 10) in RNA preparation. |
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| Keywords: | oligo(U) sequences translational control RNA Taka-diastase Author to whom all correspondence should be addressed |
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