Optimization of glycerol feeding for clavulanic acid production by Streptomyces clavuligerus with glycerol feeding |
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Authors: | Kuo-Cheng Chen Yun-Huin Lin Cheng-Min Tsai Chen-Hao Hsieh Jer-Yiing Houng |
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Institution: | (1) Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan;(2) Department of Chemical Engineering, I-Shou University, Kaohsiung County, Taiwan |
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Abstract: | Glycerol at 10–20 g l–1 increased clavulanic acid production by Streptomyces clavuligerus in shake-flask culture. The biosynthesis of clavulanic acid continued for longer by feeding glycerol and production increased to 250 mg l–1 compared with 115 mg l–1 without feeding. In fermenter batch culture, degradation of clavulanic acid began after 72 h. With glycerol feeding in fed-batch culture, clavulanic acid production was not only increased further to about 280 mg l–1 but also remained stable up to 130 h. |
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Keywords: | clavulanic acid feeding glycerol Streptomyces clavuligerus |
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