A novel set of 223 EST-SSR markers in <Emphasis Type="Italic">Casuarina</Emphasis> L. ex Adans.: polymorphisms,cross-species transferability,and utility for commercial clone genotyping |
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| Authors: | Xiuyu Xu Changpin Zhou Yong Zhang Weiqiang Zhang Xianhua Gan Huaxin Zhang Yong Guo Siming Gan |
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| Institution: | 1.Guangdong Key Laboratory of Forest Silviculture and Utilization,Guangdong Academy of Forestry,Guangzhou,China;2.Research Center of State Forestry Administration on Saline and Alkali Land,Chinese Academy of Forestry,Beijing,China;3.Key Laboratory of State Forestry Administration on Tropical Forestry Research, Research Institute of Tropical Forestry,Chinese Academy of Forestry,Guangzhou,China;4.Guangzhou Geneunion Biotechnological Company Ltd,Guangzhou,China |
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| Abstract: | Simple sequence repeat (SSR) markers are very useful for genetic applications in plants, but SSR resource for the important tree genus Casuarina L. ex Adans. is still limited. In this study, we report a novel set of 223 SSR markers in Casuarina developed from expressed sequence tag (EST) resource of GenBank. The 223 EST-SSR markers were polymorphic among 10 unrelated individuals of C. equisetifolia L. Johnson, with the number of alleles per locus (Na), observed heterozygosity (Ho), expected heterozygosity (He), and polymorphic information content (PIC) averaging at 5.5, 0.72, 0.86, and 0.63, respectively. The rates of cross-species transferability ranged from 96.9% (C. glauca Sieber ex Sprengel) through 97.8% (C. cunninghamiana Miquel) to 99.1% (C. junghuhniana Miquel). Fifty-five C. equisetifolia clones widely planted in China were successfully genotyped with a subset of 20 EST-SSRs. These newly developed markers will have a great potential for genetic and breeding applications in Casuarina species and related taxa. |
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