Fractionation of the gene-size macronuclear chromatin fragments of the binucleated eukaryote Oxytricha |
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| Authors: | Thomas J. Laughlin Adría L. Herrmann Donald E. Olins |
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| Affiliation: | (1) University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences and Biology Division, Oak Ridge National Laboratory, 37830 Oak Ridge, TN, USA;(2) Present address: Southern Research Institute, Post Office Box 55305, 35255 Birmingham, AL, USA;(3) Biology Division, Oak Ridge National Laboratory, P.O. Box Y, 37831 Oak Ridge, TN, USA |
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| Abstract: | Summary The macronuclear chromatin of Oxytrichia nova consists of chromatin fragments which are fully soluble in 0.2 mM EDTA and whose DNA length varies from 500–25 000 bp. The DNA migrates electrophoretically as a series of discrete bands, with specific genes present in only one or a few bands. The chromatin fragments are composed of nucleosomes and migrate electrophoretically in proportion to their DNA length. These results suggest schemes for the fractionation of undigested chromatin in order to enrich for specific genes, facilitating analysis of changes in chromatin structure associated with changes in gene expression. |
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| Keywords: | chromatin fractionation hypotrichous ciliate protozoa |
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