AgNO3对网纹甜瓜试管苗糖代谢及抗氧化酶活性的影响

在网纹甜瓜继代培养过程中加入不同浓度的AgNO3，测定试管苗糖代谢及抗氧化酶活性等。结果表明，玻璃苗果糖、葡萄糖和可溶性糖含量显著降低，而蔗糖含量显著高于正常苗；抗氧化系统发生紊乱，O2产生速率降低，H2O2含量下降，MDA含量增加，木质素含量降低。加入30μmol·L^-1AgNO，使玻璃苗蔗糖合成酶、蔗糖转化酶活性和木质素含量与正常苗差异不显著。说明适当浓度AgNO3有利于维持试管苗糖代谢正常进行，降低试管苗脂质过氧化程度，从而降低或抑制玻璃化现象的发生。

Effects of AgNO_3 on Sugar-Metabolizing and Antioxidant Enzyme Activities of Netted Melon(Cucumis melo L.) in vitro

Different concentrations of AgNO3 were added to the media in the process of subculture of netted melon （Cucumis melo） ＇Zhongmi 1 ＇, and sugar metabolism and antioxidant enzymes activities of plantlet were determined. The results showed that the contents of fructose, glucose and soluble sugar of vitrified plantlet decreased significantly, and the sucose content was significantly higer than that in the normal. The activities of antioxidant enzymes disordered in the vitrified plantlets, the introduction rate of O2 and H2O2 content were lower and MDA content was higher than those in the normal; and the lignin content also decreased. There were no significant differences of the ligin content and activities of sucrose synthase and invertase between vitrified and normal plantlets with treatment of 30 μmol.L^-1 AgNO3. This illustrated that proper concentration ofAgNO3 was propitious to maintain sugar metabolism, slow down lipid peroxidation, and resulted in reduction or inhibition of the vitrification.