Mitochondrion-rich cells in the embryonic yolk sac of Oreochromis mossambicus |
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| Authors: | H C Lin ¶ P P Hwang †‡ T H Lee § |
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| Institution: | Department of Biology, Tunghai University, Taichung, Taiwan, Republic of China;Institute of Zoology, Academia Sinica, Taipei, Taiwan, Republic of China;Institute of Fisheries Science, National Taiwan University, Taipei, Taiwan, Republic of China;Department of Zoology, National Chung-Hsing University, Tachung, Taiwan, Republic of China |
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| Abstract: | By removing a small amount of yolk, tilapia embryos were dechorionated successfully as early as 30 h after fertilization. Using DASPEI, a mitochondrion-specific fluorescent stain, we were able to determine the first appearance of the mitochondrion rich cells on the surface of the yolk sac 26 h after fertilization ( c. 2 h after the beginning of gastrula stage). However, with scanning electron microscopy examination, no apical crypt could be found until 48 h after fertilization. |
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| Keywords: | tilapia embryo Oreochromis mossambicus DASPEI chloride cells MR cells osmoregulation |
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