A minimal view of single-particle imaging with X-ray lasers |
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Authors: | N. Duane Loh |
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Affiliation: | 1.Center for Bioimaging Sciences and Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117411;2.PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA |
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Abstract: | The ability to serially interrogate single biomolecules with femtosecond X-ray pulses from free-electron lasers has ushered in the possibility of determining the three-dimensional structure of biomolecules without crystallization. However, the complexity of imaging a sample''s structure from very many of its noisy and incomplete diffraction data can be daunting. In this review, we introduce a simple analogue of this imaging workflow, use it to describe a structure reconstruction algorithm based on the expectation maximization principle, and consider the effects of extraneous noise. Such a minimal model can aid experiment and algorithm design in future studies. |
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Keywords: | X-ray free-electron laser coherent diffraction single particle imaging bioimaging |
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