Fermentative lactic acid production with a metabolically engineered yeast immobilized in photo-crosslinkable resins |
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| Institution: | 1. Department of Chemical Engineering and Centre for Integrated Bioprocessing Research, University of Bath, Claverton Down, BA2 7AY, Bath, UK;2. School of Life & Health Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK;3. Safety and Environmental Assurance Centre, Unilever, Colworth Park, Sharnbrook, Bedfordshire, MK44 1LQ UK;1. MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science & Engineering, Zhejiang University, Hangzhou 310027, China;2. Beijing Key Laboratory for Green Catalysis and Separation, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, China;3. R&D Center for Membrane Technology, Department of Chemical Engineering, Chung Yuan University, Chung-Li 32023, Taiwan;1. Fudan University, Affiliated Pudong Medical Center & Pharmaceutical Analysis Department, School of Pharmacy, Fudan University, Shanghai, 201203, China;2. School of Sports Science and Engineering, East China University of Science and Technology, Shanghai, 200237, China;3. Phenom Scientific Instrument (Shanghai) Co., Ltd., Shanghai, 201203, China;1. State Key Laboratory of Separation Membranes and Membrane Process, Tianjin Polytechnic University, Tianjin 300387, China;2. School of Material Science and Engineering, Tianjin Polytechnic University, Tianjin 300387, China;3. School of Environmental and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300387, China;1. Department of Chemical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia;2. Advanced Membrane Technology Research Centre (AMTEC), Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia |
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| Abstract: | In this study, the immobilization technique involving photo-crosslinkable resin gels was used for lactic acid production. Saccharomyces cerevisiae OC-2T T165R, a metabolically engineered yeast that produces optically pure l(+)-lactic acid, was immobilized in hydrophilic photo-crosslinked resin gels as a biocatalyst. Three resin gels, TEP 1, TEP 2 and TEP 3, were examined and all of them showed high performance as to lactic acid production. Resin gel TEP 1, which exhibited the highest productivity among the resin gels was used for 15 consecutive batch fermentations without decreases in productivity and mechanical deformation, indicating that it was a suitable carrier for long-term lactic acid fermentation. Moreover, the use of the immobilization technique can improve the productivity of the metabolically engineered yeast in the fermentation with or without extraction, showing promise for using the immobilized engineered yeast for lactic acid production. |
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