Refractive index of uranyl-treated bacterial cytoplasm as related to ribonucleic-acid content and growth rate |
| |
Authors: | G W Fuhs M Chen |
| |
Institution: | 1.Division of Laboratories and Research,New York State Department of Health,Albany |
| |
Abstract: | After fixation and treatment with uranyl acetate solutions, the refractive index (n D) of the cytoplasm ofCorynebacterium bovis varies with substrate-dependent growth rate and RNA content. The effect, which presumably is due to quantitative binding of uranyl ions by RNA, permits a measurement of the growth rate of single cells in a single-species system by interference microscopy. Temperature-induced changes in growth rate are not reflected in changes ofn D or RNA content. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|