Studies on the use of a thermotolerant strain of Kluyveromyces marxianus in simultaneous saccharification and ethanol formation from cellulose |
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Authors: | N Barron R Marchant L McHale A P McHale |
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Institution: | (1) School of Applied Biological and Chemical Sciences, University of Ulster, Coleraine, Co. Londonderry, BT52 1SA, Northern Ireland, IE |
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Abstract: | The thermotolerant yeast strain, Kluyveromyces marxianus IMB3, was found to be capable of ethanol production during growth at 45°C on media containing milled paper and exogenously
added commercial cellulase. At maximum achievable cellulose concentrations in shake-flask cultures, ethanol production increased
to 6.6 g/l at 45°C, representing an overall level of conversion of 21% of the maximum theoretical yield. Subsequent studies
involving variations in added cellulase concentrations to the batch systems demonstrated that ethanol yields could be increased
to 10 g/l at 45°C, which represented 39% of the maximum theoretical yield. As a result of ethanol production at 45°C in the
systems examined, we suggest that the thermotolerant ethanol-producing yeast strain K. marxianus represents a novel candidate for use in simultaneous saccharification and conversion of the resulting substrates to ethanol.
Received: 9 June 1994/Received revision: 8 August 1994/Accepted: 12 August 1994 |
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