Functional polymeric supports for immobilization of cholesterol oxidase |
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| Affiliation: | 1. Department of Chemistry, Faculty of Art and Science, Gazi University, 06500 Besevler, Ankara, Turkey;2. Department of Chemical Engineering, Hacettepe University, 06800 Beytepe, Ankara, Turkey;1. Institute of Chemistry, St.Petersburg State University, 198504, St.Petersburg, Russia;2. International Laboratory of Nonlinear Optical Informatics, ITMO University, 197101, St.Petersburg, Russia;3. Institute of Macromolecular Compounds, Russian Academy of Sciences, 199004, St.Petersburg, Russia;1. Department of Clinical Laboratory, Linyi People''s Hospital, LinYi 276003, China;2. Department of Obstetrics, General Hospital of Laiwu Iron and Steel Company, Laiwu 271126, China;3. Department of Proctology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China |
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| Abstract: | Here, we have reported the useful functional polymeric supports for possible application of enzyme immobilization. Functional polymers were prepared by free radical polymerization from different monomers (i.e., methylmetacrylate, glycidylmethacrylate, acrylamide, etc.) and N,N-methylenebis(acrylamide) (MBAAm) crosslinker. Cholesterol oxidase (ChOx) [EC.1.1.3.6] was then covalently immobilized onto these functional supports via epichlorohydrin (ECH) and carbodiimide (EDAC) as the activating agents. It was observed that, after 60th use in 5 days, the retained activities for immobilized enzymes onto poly(methyl methacrylate-co-glycidyl methacrylate) [P(MMA-co-GMA)] and poly(acrylamide-co-acrylic acid)/polyethyleneimine [P(AAm-co-AA)/PEI] supports were found as 56% and 83%, respectively. |
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