The effect of pH on the oxygen kinetics of cytochrome c oxidase proteoliposomes
Authors:
Lars Chr. Petersen
Affiliation:
Institute of Biochemistry, Odense University, Campusvej 55, 5230, Odense M, Denmark
Abstract:
The effect of pH on the oxygen kinetics of cytochrome c oxidase incorporated into phospholipid vesicles is studied. The pH profiles of the oxygen kinetics of energized and deenergized oxidase vesicles are similar. An effect of pH on the slope of the reciprocal plot of rate against oxygen concentration is observed, and this may indicate that protons are involved in the rate limiting step of the reaction between oxygen and reduced oxidase. In contrast to the pH dependence of the oxygen kinetics, the binding of CO to the oxidase is not pH dependent.