Iodine speciation in human serum by reversed-phase liquid chromatography-ICP-mass spectrometry |
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Authors: | Bernhard Michalke Peter Schramel Heidi Witte |
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Institution: | (1) GSF National Research Center for Environment and Health, Institute for Ecological Chemistry, Ingolstaedter Landstr. 1, D-85764 Neuherberg, Germany |
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Abstract: | Reversed-phase liquid chromatography-inductively coupled plasma mass spectrometric hyphenation was used for iodine speciation
in human serum. First investigations showed that iodine species nearly quantitatively were eluted in the void volume. The
result indicated that protein-linked thryoid hormones were not interacting with the stationary phase, thus being not retained.
Investigations were performed about T4-TBG (thyroxin-thyroxin-binding globulin) complex generation and its retention during
chromatography. It was shown that T4-TBG was not retained on the column. Therefore, a protease treatment was introduced for
serum sample preparation. The analysis of “normal” sera (after protease) gave reasonable results lying in the range published
in literature: I-:11; di-iodothyrosine (DIT): 2.1; mono-iodothyrosine (MIT): 1.6; reversed tri-iodothyronine (rT3): 3.9; T3:
5.9; T4: 60; each micrograms iodine per liter. The method also proved to recognize abnormalities in a pathologic serum, having
rT3 as the predominant species. In this case the method obviously was superior compared to standard immunoassay methods, as
it is monitoring the iodine in the species (physiologically active iodine species), whereas immunoassay methods may sometimes
detect deiodinated (inactive) compounds. |
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Keywords: | Iodine speciation RPC-ICP-ITS serum thyroid hormones |
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