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Visualization of anti-histone antibodies on SV40 minichromosomes by scanning transmission electron microscopy (STEM)
Authors:D Ghose  E Weiss  J C Homo  M H Van Regenmortel  P Oudet
Affiliation:1. U184 de Biologie Moléculaire et de Géne Génétique de l''INSERM, Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Faculté de Médecine, F-67085 Strasbourg Cédex France;2. IBMC, 15, rue Descartes, F-67000 Strasbourg, France
Abstract:The unique capabilities of the scanning transmission electron microscope (STEM) have been used for a high resolution study of antibody binding to individual SV40 minichromosomes. A method of sample preparation has been developed which allows direct visualization of the antibody molecules in a clearly recognizable form. Using this technique, we have studied the binding of anti-H2B and anti-H3 immunoglobulins to SV40 minichromosomes. The results indicate that histones H2B and H3 are located only in the nucleosomes and are absent in the linker regions.
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