|
|||||
|
|
Characterization of two novel antimicrobial peptides from the cuticular extracts of the ant Trichomyrmex criniceps (Mayr), (Hymenoptera: Formicidae) |
|
| Authors: | Nagachaitanya Bhagavathula Venkateshwarlu Meedidoddi Simon Bourque Reshmy Vimaladevi Santoshkumar Kesavakurup Dayanandan Selvadurai Sameer Shrivastava Chandrashekara Krishnappa |
| Institution: | 1. Department of Applied Zoology, School of Biological Sciences, Kuvempu University, Shivamogga, Karnataka, India;2. Department of Agricultural Entomology and School of Ecology & Conservation, University of Agricultural Sciences, Bengaluru, Karnataka, India;3. Department of Biology and Centre for Structural and Functional Genomics, Concordia University, Montreal, Quebec, Canada;4. Department of Chemical Biology, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India;5. Veterinary Biotechnology Division, Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India |
| Abstract: | Antimicrobial peptides (AMPs) from cuticular extracts of worker ants of Trichomyrmex criniceps (Mayr, Hymenoptera: Formicidae) were isolated and evaluated for their antimicrobial activity. Eight peptides ranging in mass from 804.42 to 1541.04 Da were characterized using a combination of analytical and bioinformatics approach. All the eight peptides were novel with no similarity to any of the AMPs archived in the Antimicrobial Peptide Database. Two of the eight novel peptides, the smallest and the largest by mass were named Crinicepsin‐1 and Crinicepsin‐2 and were chemically synthesized by solid phase peptide synthesis. The two synthetic peptides had antibacterial and weak hemolytic activity. |
| Keywords: | antimicrobial activity antimicrobial peptides hemolytic activity solid‐phase peptide synthesis Trichomyrmex criniceps |